O'Sullivan, who had already won the frame, stayed composed to sink the pink while the invader was causing a ruckus, and was one ball away from a total clearance of 136 points.
But rather than pot the black himself he handed his cue to the giddy fan, who was dressed in blue jeans and a grey top and carrying an orange handbag.
"I saved her from getting a rugby tackle," O'Sullivan said.
And O'Sullivan moved into the fourth round by beating Chinese player Zhang Yong 4-1 at the Barnsley Metrodome in northern England on Thursday.