There to officiate at the event included President of the PNG Olympic Committee, Sir John Dawanincura, Trukai's National Marketing Manager, Maybellyne Fernandes, PNG's super fish, Ryan Pini and Team PNG Chef de Mission to Rio, Emma Waiwai.
This year's event is expected to see over 50,000 participants hit the streets to take part in the nationwide event on June 26, 2016.
The PNG Cancer Foundation has also joined in as a partner for the Fun Run with Patron, Lynda Baboa-O'Neill present to launch the Fun Run shirts.
Dawanicurra said all funds raised from the Fun Run will again go to sending Team PNG to compete in international events, with the focus this year being centred around the 2016 Rio Olympics.
He said the success of Team PNG can be attributed to the support from Trukai and other support sponsors including the government.
Waiwai added encouraging people to show their support for Team PNG by buying a shirt and participating.
Fernandes said Trukai was excited to host the annual event that promotes good health and an active lifestyle.
She said preparations have been going well.
"We are sponsoring 100, 000 shirts again as our contribution to the fundraising drive for Team PNG.
"While we are yet to confirm figures, we are confident the number of participating centres will increase from last year's 16," said Fernandes.
The auction for this year's Fun Run will take place in Lae on the 16th of March at the Lae Yacht Club and the 17th at the Royal Papua Yacht Club.