The companies include Exxon Mobil, Kwila Insurance, ANZ Bank and Pacific MMI Insurance, which was the first to register.
The annual charity golf challenge is organised by the Papua New Guinea Cancer Foundation.
It was launched on June 21 in Port Moresby with registration open to corporate companies and individual groups.
Over 30 teams are expected to take part with early bird registration fee of K3, 000. Those that register as of August 1 will pay a fee of K3, 500.