The world's largest fish market, which has been in operation for 83 years, is being moved as part of the redevelopment for the 2020 Olympic Games.
The famed tuna auctions are a popular tourist attraction in the Japanese capital.
"I'm almost crying," said retired seafood auctioneer Hisao Ishii, who returned to the market for its last day on Saturday.
"Today is a sad day of goodbyes. Tsukiji tried to meet the times, but it is getting older," he told the AFP news agency.