The Jamaican icon, an eight-time Olympic champion with 11 world titles, will hang up his spikes following the championships and clocked a time of 10.07 seconds to advance to Saturday's semi-finals.
Bolt bowed to the fans before setting off in the sixth and final heat, but only crossed the line first - seemingly leaving plenty in the tank - after starting sluggishly.
It was the eighth-fastest time of the night and the 30-year-old was clearly unimpressed, shaking his head after looking at the replay on the big screen.
