The event was dubbed 'Salute to a Legend' as eight-time Olympic gold medallist and world-record holder Bolt bade farewell to his compatriots on the track.
Naturally, Bolt won - and in typical style too, bursting clear in the race's final phase - crossing the line in 10.03 seconds, as fireworks greeted the sprinters at the finish.
The 30-year-old will retire after this year's World Championships, looking to swell his collection of 11 gold medals.
Usain Bolt does his signature pose for last time as a competitor in Jamaica. Your legacy will be in our hearts forever. pic.twitter.com/rGLLG9TeSV
— Team Jamaica (@JamaicaOlympics) June 11, 2017
Fellow athletes David Rudisha and Mo Farah were there to celebrate with Bolt - Farah won the 3000m event - and Bolt was in a reflective mood after his victory, which follows the death of his close friend, former Team GB sprinter Germaine Mason.
He is quoted by the Jamaica Gleaner: "After my friend's death it was really hard for me, I have never been through something like this.
"I had to take some time but I knew what I had to do and I knew Germaine would want me to do this. This was special.
"There are no words, from World Juniors the support that I have been getting from Jamaica has been remarkable.
"I could not have done this without my parents, my friends, my best friend NJ, I want to thank Jamaica, I never expected this.
"It's big to see everybody turn out, it shows that they appreciated what I did and it was a honour for me."
Thank you Jamaica
— Usain St. Leo Bolt (@usainbolt) June 11, 2017
Photo: Twitter (@JamaicaOlympics)- Usain Bolt celebrates his 'Salute to a Legend' victory in Kingston