They were caught by MS 11 from Wabag at around 4.00pm along the Nanonda Highway.
The MS 11 had been task to conduct police road blocks along the highway when they incepted the two suspects.
Assistant Commissioner for Police- Western Command, Martin Lakari said the suspects were apprehended have all been detained at Tari police cell and refused bail.
They were formally charged on Wednesday for the alleged offence.
The unlicensed pistol is a Magnum, with Serial number: 1911A2 and is believed to be a US Army issued.
Lakari said there have been a lot of other ammunitions confiscated including Bush knives and axes.
This is the second time police have confiscated and apprehend suspects for the similar pistols.
Further updates will be made available as the joint security forces operation progresses.