Since 29 August 2015, the group lived in close quarters in a dome, without fresh air, fresh food or privacy.
Experts estimate that a human mission to the Red Planet could take between one and three years.
The Nasa-funded study run by the University of Hawaii is the longest of its kind since a Russian mission that lasted 520 days.
"The researchers are looking forward to getting in the ocean and eating fresh produce and other foods that weren't available in the dome", said Kim Binsted, principal investigator for the Hawaii Space Exploration Analog and Simulation.
The team consisted of a French astro-biologist, a German physicist and four Americans - a pilot, an architect, a journalist and a soil scientist.
The experiment dealt with the human element of exploration.
Whilst conducting research, the six had to live with limited resources, wear a space-suit when outside the dome, and work to avoid personal conflicts.