The 1972 single beat classics by the Jackson 5, Marvin Gaye and Tammi Terrell and the Temptations.
The list of the top 100 Motown tracks was compiled by the Official Chart Company based on all time downloads and streams.
"I am proud to have been chosen at the top of such an incredibly talented group of artists," said Wonder.
Overall the singer-songwriter legend had the most songs on the list with 20.
His next most popular track was Signed, Sealed, Delivered (I'm Yours), which came in at five.
"Motown is one of the all-time great labels, and I've been associated with it all my life. Many of the artists have been my friends and family," said Wonder.
"I am proud to have been chosen at the top of such an incredibly talented group of artists."
The top 10 includes two Jackson 5 songs: I Want You Back at number two and ABC at number seven. Marvin Gaye and Terrell's classic Ain't No Mountain High Enough is at number three.
From the top 10, Ne-Yo, aged 39, stands out as the only younger artist. His track Let Me Love You (Until You Learn To Love) in 2012 followed an earlier track he wrote for the singer Mario with the shorter title Let Me Love You.
In 2012 he became vice president of Motown with a role incorporating producing, songwriting and mentoring the label's artists and seeking out new talent.
Motown top 10
1. Superstition - Stevie Wonder
2. I Want You Back - Jackson 5
3. Ain't No Mountain High Enough - Marvin Gaye and Tammi Terrell
4. Let Me Love You (Until You Learn To Love) - Ne-Yo
5. Signed Sealed Delivered (I'm Yours) - Stevie Wonder
6. My Girl - The Temptations
7. ABC - Jackson 5
8. All Night Long (All Night) - Lionel Richie
9. Let's Get It On - Marvin Gaye
10. I Heard It Through The Grapevine - Marvin Gaye